ABSTRACT
Objective: To determine the role of glutathione S-transferase [GST] isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort
Subjects and Methods:In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs
Results:Of the 150 patients with psoriasis, 83 [79%] were identified with the GSTT1 genotype and 22 [21%] with the null genotype. Of the 102 patients in the control group, 69 [67.6%] subjects were identified with the GSTT1 genotype and 33 [32.4%] with the null genotype. There was no significant difference between the patient and control groups [p = 0.063]. Regarding the GSTM1 polymorphism, 54 [51.4%] patients were identified with this genotype and 51 [48.6%] with the null genotype; in the control group, 50 [49%] were identified with this genotype and 52 [51%] with the null geno- type. Again there was no statistically significant difference between the groups [p = 0.957]
Conclusion:In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed
ABSTRACT
Background and Aim: studies of associations between various cancers and the ABO blood groups have shown elevated relative risks for some categories of disease. There has so far been no report of an evaluation of the relationship between the ABO blood groups and acne vulgaris. To investigate this association, we conducted a retrospective study of acne vulgaris diagnosed in Turkey
Material and Methods: all cases were clinically confirmed. Blood information was obtained on 498 individuals with acne vulgaris, and the distribution of ABO and Rh blood type for cases was compared with that of 419 healthy blood donors from the same geographic area
Results: patients with group A and B blood groups ratios were higher than the control group, but not statistically significant [P = 0.325 and P = 0.138]. The ratio of the patient group with AB blood group was significantly higher than in the control group [P < 0.01]. The ratio of blood group O of patient group was significantly lower than in the control group [P < 0.01]. There were no statistically significant differences between the patient and control groups in the distribution of Rh factor
Conclusion: our study showed a significant association of AB and O blood groups with acne vulgaris. Further studies in a larger series on blood group antigens are needed to shed some light on the relationship between these antigens and skin cancer
ABSTRACT
To investigate the role of human leukocyte antigen [HLA] in susceptibility to psoriasis vulgaris in the Northeast region of Turkey and to contribute to the data related to HLA and psoriasis. The study included 72 unrelated psoriatic patients [43 men and 29 women; aged 11-76 years] admitted to the Dermatology Department, University Research Hospital, Erzurum, Turkey between April 2002 and November 2003. We studied the distribution of HLA class I and II antigens in patients with psoriasis: 72 patients were divided into 2 groups according to the onset of psoriasis before age 40 years with family history [type I] and onset after age 40 without family history [type II]. The HLA class I and II antigens were analyzed using the PCR-SSP method in 72 patients and in 104 controls. We found an increase in HLA-A30 and A68, B7, Bl3, B57,Cw6, and DRB 107 antigens in psoriatic patients compared with controls. As we compared type I and type II psoriasis with control group, B57, Cw6 and DRB 107 alleles were more significant in patients with type I psoriasis. Our patients with type II psoriasis represented a significant association with the HLA-B13. Our findings along with previous HLA studies on psoriasis vulgaris patients from different racial groups showed that HLA-B57 and DRBI 07 alleles are associated with the disease
Subject(s)
Humans , Male , Female , Alleles , /genetics , Psoriasis/classification , Polymerase Chain Reaction , Case-Control StudiesABSTRACT
Lichen planus (LP) is a common, pruritic and inflammatory disease of the skin, hair follicles and mucous membranes. Immunologic mechanisms, especially cell-mediated immunity, play a major role in triggering the clinical expression of the disease. P-selectin is an adhesion molecule present within endothelial cells and mediates endothelial-leukocyte interactions. Therefore, it is considered that P-selectin plays an important role in LP. The aim of our study is to research the relation between P-selectin and LP. Serum P-selectin levels were determined with the enzyme- linked immunosorbent sandwich assay method in sera from 40 LP patients and 40 healthy controls. The serum levels of P-selectin were statistically significantly higher in the patients than in healthy controls (p < 0.05), in female patients (39.32 +/- 11.34 pg/ml) than in male patients (31.93 +/- 9.83 pg/ml) (p < 0.01), and in the patients with eruptive form (40.27 +/- 9.32 pg/ml) than in those with the localised (32.83 +/- 9.93 pg/ml) and hypertrophic (31.72 +/- 8.39 pg/ml) forms (both p < 0.01). In conclusion, we found a meaningful relation between LP and serum P-selectin levels.